Journal: Purinergic Signalling

Article Title: Modulation of G protein-coupled adenosine receptors by strategically functionalized agonists and antagonists immobilized on gold nanoparticles

doi: 10.1007/s11302-012-9338-z

Figure Lengend Snippet: AR binding affinity of AuNP conjugates and intermediates

Article Snippet: Synthesis When the same procedure as for 21b (except redissolving the crude product in a water/methanol mixture (75:25)) was carried out using a 20:1 ratio of hydrogen tetrachloroaurate(III) tetrahydrate to each monomer 13 and 16 , a larger AuNP conjugate, 21a , was obtained. . Purification process The deep red-colored solution of the crude complex was filtered using a Millipore Amicon Ultra-15 centrifugal filter (30 K MW cutoff) and the solid remaining on the filter redissolved for five cycles.

Techniques: Binding Assay, Inhibition

Journal: Purinergic Signalling

Article Title: Modulation of G protein-coupled adenosine receptors by strategically functionalized agonists and antagonists immobilized on gold nanoparticles

doi: 10.1007/s11302-012-9338-z

Figure Lengend Snippet: Coupling of AR agonists 13 and 17 and antagonists 14 and 18 to AuNPs. For the A3AR agonist, both large diameter 21a (4–5 nm) and smaller diameter 21b (~2 nm) conjugates were prepared. Reagents and conditions: a HAuCl4, NaBH4, DMF

Article Snippet: Synthesis When the same procedure as for 21b (except redissolving the crude product in a water/methanol mixture (75:25)) was carried out using a 20:1 ratio of hydrogen tetrachloroaurate(III) tetrahydrate to each monomer 13 and 16 , a larger AuNP conjugate, 21a , was obtained. . Purification process The deep red-colored solution of the crude complex was filtered using a Millipore Amicon Ultra-15 centrifugal filter (30 K MW cutoff) and the solid remaining on the filter redissolved for five cycles.

Techniques: